SubtiBank SubtiBank
yxjG [2018-02-12T17:33:57.000Z]
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yxjG [2018-02-12T17:33:57.000Z]

putative B12-independent methionine synthase
locus
BSU38960
pI
5.01
mw
42.96 kDa
protein length
378 aa Sequence Blast
gene length
1134 bp Sequence Blast
function
unknown
product
putative methionine synthase
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.11|Biosynthesis/ acquisition of methionine/ S-adenosylmethionine/ based on similarity]

    • Gene

    Coordinates
    3,999,350 → 4,000,486

    The protein

    Paralogous protein(s)

  • [protein|55459D8F0BBB2669EB9F25276A17F1A7EB322AF1|YxjH]

    • Modification

  • S-bacillithiolation upon hypochlorite stress on Cys-346 [Pubmed|21749987]

    • Structure

  • [PDB|1YPX] (the enzyme from ''Listeria monocytogenes'', 49% identity)

    • Expression and Regulation

    Operons

    genes
    [gene|A866ACE8431EC3FF98ADC734D9322AE699ECBFAD|yxjG]
    description
    [Pubmed|10746760]

    regulatory mechanism

  • [regulon|S-box|S-box]: termination, the [SW|S-box] [SW|riboswitch] binds S-adenosylmethionine resulting in termination [Pubmed|10094622], in [regulon|S-box|S-box]

    • regulation

  • induced by methionine starvation ([SW|S-box]) [Pubmed|10094622]
  • the [SW|S-box] RNA is degraded by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [pubmed|29794222]
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    Biological materials

    Mutant

  • MGNA-B731 (yxjG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1730 NBRP B. subtilis, Japan]
  • BKE38960 (Δ[gene|A866ACE8431EC3FF98ADC734D9322AE699ECBFAD|yxjG]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE38960 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTGTCCATTCCTTCTT, downstream forward: _UP4_TAAGCAAAGAAAAAAACACA
  • BKK38960 (Δ[gene|A866ACE8431EC3FF98ADC734D9322AE699ECBFAD|yxjG]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK38960 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTGTCCATTCCTTCTT, downstream forward: _UP4_TAAGCAAAGAAAAAAACACA

    • References

  • 19258532,10094622,18039762,12107147,21749987